anti-hER-α36 antibody 抗體    

產品說明

Descriptions: The antibody was raised against a synthetic peptide antigen corresponding to the unique C-terminal 20 amino acids of hER-α36. The specificity of the antibody was tested in hER-α36 expression vector transfected HEK293 cells that do not express endogenous hER-α. Immunofluorescence was used to demonstrate immunoreactive signals only in transfectants with the hER-α36-expressing vectors but not in transfectants harboring an empty expression vector. This rabbit polyclonal anti-hER-α36 antibody is supplied in affinity-purified form.

Storage: 2-8 ℃ for short term; freeze in Suitable Aliquots.

Antibody Concentration: 1 mg/ml.

Physical Appearance: Liquid.

Buffer: Tris/Glycine (pH7.4).

Stabilizing Agent: 0.1% BSA.

Preservative: in 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide.

Shelf Life: 6 months from date of receipt.

Reactivity: human, mouse, and hamster (not rat ,others not tested)

Application: immunostaining, westernblot, immunoprecipitation.

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  anti-hER-α36 antibody 抗體    

論文引用（2010-2019部分）



1.Estrogen signaling and estrogen receptors as prognostic indicators in laryngeal cancer
A Verma, N Schwartz, DJ Cohen, BD Boyan… - Steroids, 2019 - Elsevier
… Abcam, Cambridge, United Kingdom) or ERα (rabbit polyclonal, Chi Scientific, Maynard, MA),
which detects ERα66 and ERα36. All tissue or cell lysates were run on two separate western
blots, and data from one representative blot is shown. 2.8.1. Estrogen and estrogen …
 

2.24R, 25-dihydroxyvitamin D3 modulates tumorigenicity in breast cancer in an estrogen receptor-dependent manner
A Verma, Z Schwartz, BD Boyan - Steroids, 2019 - Elsevier
… Western blots were used to identify and quantify estrogen receptor protein in all cell lines as
previously described [9]. In brief, cells were grown to confluence in T …ERα66, ERα46, and ERα36
were assessed with an all-ERα primary antibody (Chi Scientific #80113, Maynard, MA) …

3.Effect of 17β-estradiol on estrogen receptor negative breast cancer cells in an osteolytic mouse model
DJ Cohen, V Patel, A Verma, BD Boyan, Z Schwartz - Steroids, 2019 - Elsevier
… Effects of estrogen on osteolysis were assessed as a function of time and treatment with
17β-estradiol. 2. Material and methods … Primary antibodies against ERα were from Chi Scientific
(Maynard, MA, #8-80119, #8-80113); antibodies to GAPDH were from Millipore-Sigma (St …
 

4.Estradiol receptor profile and estrogen responsiveness in laryngeal cancer and clinical outcomes
N Schwartz, A Verma, C Muktipaty, C Bivens… - Steroids, 2019 - Elsevier
… Louis, MO, #C4555-10G) [16]. Estrogen receptors were inhibited using a neutralizing antibody
to ERα36 (Chi Scientific, Maynard, MA) [37]. Confluent cultures were pre-treated with media
containing different concentrations of the inhibitor as indicated below …
 

5.Estrogen receptor-alpha 36 mediates the anti-apoptotic effect of estradiol in triple negative breast cancer cells via a membrane-associated mechanism
RA Chaudhri, A Hadadi, KS Lobachev… - … et Biophysica Acta (BBA …, 2014 - Elsevier
… Estrogen receptors (ERs) play a major role in classification, diagnosis, and treatment of breast
cancer [13], [14], [15] … ERα36 overexpression plasmids were purchased from Chi Scientific
(Maynard, MA). Polyfect transfection reagent was obtained from Qiagen (Germantown, MD) …

6.Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36)
RA Chaudhri, R Olivares-Navarrete, N Cuenca… - Journal of Biological …, 2012 - ASBMB
… needed. The purpose of this study was to evaluate the role of ERα36 in membrane-
associated estrogen signaling in breast cancer … Sciences. Polyclonal ERα66 and ERα36
antibodies were purchased from Chi Scientific (Maynard, MA) …
 
7.17β-Estradiol regulates rat growth plate chondrocyte apoptosis through a mitochondrial pathway not involving nitric oxide or MAPKs
M Zhong, DH Carney, BD Boyan, Z Schwartz - Endocrinology, 2011 - academic.oup.com
… The effects of estrogen on proliferation and differentiation of rat costochondral resting zone (RC)
chondrocytes involve a membrane … Anti-ERα36 antibody was purchased from Chi Scientific
(Maynard, MA); 3,3′-diaminobenzidine substrate was purchased from Roche Applied …
 

8.Apoptotic signaling pathways in mammalian growth plate chondrocytes
M Zhong - 2010 - smartech.gatech.edu
… E2-induced apoptosis were also assessed. We also assessed the roles of different estrogen
receptors in E2-induced apoptosis. Page 25. 14 Chapter 2 … Anti-ERα36 was purchased from Chi Scientific (Maynard, MA). 3,3′-diaminodbenzidine (DAB) substrate was …


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